Lipidure®-CM5206

Incoterms: EX-Works NOF's warehouse in Japan.

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－Features－
-Hemo-compatibility
-Anti-adhesion of proteins and cells
-Anti-denaturing of proteins
-Anti-activation of cells

－Usage－
-Dissolve Lipidure®-CM in ethanol and prepare a 0.5wt% ethanol solution.
-Dip the base material into the coating solution for 1 min.
-Dry the coated material at room temperature or one hour at 50°C.

－Substrates－
-Lipidure®-CM can be coated on the following substrates:
-Plastics (PET, PMMA, PSt, PU, PC, PE, PP)
-Glass, Ceramics and Metals (SUS, titanium, gold)

－Comparison of results－

Protein Adsorption

	Experimental procedure -Dispensation of HRP-IgG conjugate solution into each well -Incubation for 1 h at 25ºC -Washing three times with Dulbeco’s PBS including 0.05% of Tween20. -Dispensation of 3,3’,5,5’-tetramethylbenzidine solution -Measurement of absorbance at 450 nm
The Lipidure® treatment was able to reduce the amount of IgG absorption to below 10% of non-treated pleat. The same results were observed with other proteins.

Macrophage adhesion

	Experimental procedure -Seeding of macrophage into each well (3.3 x 105 cells/well) -Incubation for 1 h at 37ºC in 5% CO2 atmosphere -Washing with Dulbeco’s PBS -Fixing with glutaraldehyde solution -Observation by SEM
The activation and adhesion of macrophage was prevented by Lipidure® treatment. Also, the Lipidure® treatment was effective for other cells.

－Coating－
PC surface can be made by coating with Lipidure®-CM. The following figure shows the relationship between “the number of times the coating was applied” and “the thickness of the coating film”. The thickness of the Lipidure®-CM coating film is calculated by weight measurement. These data are examples in the case of dip coating. The thickness was controlled by adjusting the number of times of a coating was applied and the concentration of Lipidure® in the coating solution.

－Safety Data－
We have the following toxicological data for Lipidure®-CM5206. Acute oral toxicity, Skin irritation, Skin sensitization, Mutagenic potential (Ames Test), Hemolysis test, Endotoxin test.

Relationship between “number of times coating was applied” and “thickness of coating film”