Lipidure®-CM5206

Chemical structure

Coating Method

Available substrate

Lipidure®-CM5206 can be coated on the following materials.

✓ Glass
✓ Plastic (PET, PMMA, PSt, PU, PC, PE, PP)
✓ Metal (SUS, Titanium, Gold)
✓ Ceramic

Feature

✓ Suppression of cell and protein adsorption
✓ Suppression of cell activation
✓ Suppression of protein denature
✓ Hemo-compatibility

・Suppression of protein adsorption

Method

  1. Prepare well plate coated with Lipidure®-CM5206
  2. Add HRP-IgG conjugate solution into each well
  3. Incubate for 1 hr at 25 ºC
  4. Rinse three times with 0.05 % Tween®20 (*) /Dulbecco’s PBS
  5. Add of 3,3’,5,5’-tetramethylbenzidine solution into each well
  6. Measure absorbance at 450 nm

*Tween® is a registered trademark of Croda International PLC.

・Suppression of cell adsorption

Method

  1. Prepare well plate coated with Lipidure®-CM5206
  2. Seed macrophage in well plate at 3.3x105 cells/well
  3. Incubate for 1 hr at 37 ºC in 5% CO2 atmosphere
  4. Rinse off with Dulbeco’s PBS
  5. Fix with glutaraldehyde and Observation by SEM

・Hemo-compatibility

Method

  1. Prepare artificial vascular coated with Lipidure®-CM5206
  2. Rabbit blood was flowed in Lipidure®-CM5206 treated artificial vascular
  3. Rinse artificial vascular with buffer solution
  4. Take photo of inside of artificial vascular