SS-cleavable and pH-responsive Lipid Like, Material (COATSOME® SS-Series)

SS-cleavable and pH-responsive lipid-like material for gene, nucleic acids, and small molecule delivery system

We have newly developed COATSOME® SS-series lipids for effective intracellular delivery and lower toxicity. COATSOME® SS-series contains dual sensing motifs that can respond to the intracellular environment; tertiary amines respond to an acidic compartment (endosome/lysosome) for membrane destabilization and a disulfide bond that can cleave in the reductive environment (cytoplasm). These two units synergize for efficient intracellular delivery of encapsulated drugs with enhanced biosafety.

Proof of Concept

Membrane Disruption in Cells

The particle composed of COATSOME® SS Series is stable in serum, preventing enzymatic degradation of pDNA through encapsulation. The particle is effectively destabilized in the cytoplasmic environment.

Degradation of COATSOME® SS-OP

In vivo Toxicity Comparison

Key Features

Encapsulation: siRNA, mRNA, pDNA
Efficient cytoplasmic drug delivery
Biodegradability and low toxicity
Controlled organ targeting: liver, spleen, lymph nodes
Long-term storage of lyophilized LNP at 25℃
Compatibility with freeze-thaw processes
Vaccine applications (humoral and cellular immunity)

Examples of Application

With high biocompatibility, COATSOME® SS Series enables efficient intracellular delivery of a wide range of compounds. Particles can encapsulate pDNA, siRNA, mRNA, and hydrophobic low-molecular-weight compounds, with proven delivery to tumor, spleen, lymph node, and brain.

The following sections introduce hepatic siRNA delivery, hepatic mRNA delivery, and lymphatic mRNA delivery data for RNA vaccine applications.