LIPIDUREⓇ-CM5206, CM1102

Product Line-up

LIPIDURE®-CM5206 and LIPIDURE®-CM1102

How to use

procedures of dissolution

*LIPIDURE®-CM5206 might take time for 16hr or more for dissolution

Coating Process

Cast Coating

Performance

Suppression of platelet adsorption

Procedure

1. Dissolve LIPIDURE®-CM5206 in ethanol at 0.5 wt%.
2. Add 10 µL/well of LIPIDURE®-CM5206 soln. to 384 well tissue culture plate.
3. Remove all soln. Dry at room temperature.
4. Centrifuge rabbit whole blood. (4℃, 1,500 rpm, 20 min)
5. Collect platelet-rich plasma (PRP) and 50 µL/well.
6. Incubate at room temperature for 1 hr.
7. Remove the PRP and wash 3 times with Hanks’s buffer soln. and immobilize with 2.5% Glutaraldehyde.
8. Incubate at room temperature for 2 hr.
9. Wash with distilled water.
10. Observe under SEM after freeze drying and gold vapor deposition.

Suppression of protein adsorption

1. Dissolve LIPIDURE®-CM1102 in ethanol at 0.5 wt%.
2. Add 200 µL/well or more of LIPIDURE®-CM1102 soln.
3. Remove all soln.
4. Dry at room temperature.
5. Add 100 µL/well HRP labeled IgG conjugate soln. (24,000 times dilution by PBS).
6. Incubate at room temperature for 1 hr.
7. Wash with 0.05% Tween 20-PBS.
8. Add 100 µL/well HRP substrate soln.
9. Incubate at room temperature for 10 min.
10. Add 2N H₂SO₄ 50 µL/well to stop the HRP reaction.
11. Measure absorbance at 450 nm.

For more product information and performance date, contact us for the details.