Lipidure®-GD - Application for PCR Tests

LIPIDURE®-GD series are new additives suitable for PCR application.

Feature of LIPIDURE®-GD

Product Name Sensitivity Stability
LIPIDURE®-GD100 ++ +
LIPIDURE®-GD200 + +
LIPIDURE®-GD300 + ++
LIPIDURE®-GD400 ++ ++
LIPIDURE®-GD500 + ++

Efficacy
+ : high
++ : higher

Value

LIPIDURE®-GD will:

  • Enable a challenging detection of tiny amount of the target by PCR system.
  • Save your time to optimize your PCR system.

Function

By just putting LIPIDURE®-GD into your master mix, the PCR system gets:

  • Stronger end-point signal
  • Improved limit of detection
  • Better preservation stability.

Performance

[Sensitizing in PCR, Enhancing the end-point signal]

  1. 1 A commercially available COVID-19 RT-qPCR kit (Japanese NIID method) was prepared.
  2. LIPIDURE®-GD100 or -GD200 was added to the master mix respectively (2 vol% final as GD100, 1 vol% final as GD200).
  3. Control RNA (50 copy of N set No.2) was added.
  4. One-step RT-qPCR was conducted.

[Stabilizing master mix]

  1. A commercially available COVID-19 RT-qPCR kit (Japanese NIID method) was prepared with LIPIDURE®-GD added to the master mix.
  2. The master mix was incubated at 37°C for 2 hrs as an accelerated aging test.
  3. One-step RT-qPCR was conducted using master mix BEFORE and AFTER incubation respectively. Positive control RNA (50 copy of N set No.2) was used as template.

LIPIDURE®-GD significantly improves the preservation stability of the master mix.

For more product information and performance date, contact us for the details.