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Lipidure®-CM5206 (10g)

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Incoterms: EX-Works NOF's warehouse in Japan  ***We will send you an email for requesting your FedEx account number after receiving your order. ***


-Features-
-Hemo-compatibility
-Anti-adhesion of proteins and cells
-Anti-denaturing of proteins
-Anti-activation of cells

-Usage-
-Dissolve Lipidure®-CM in ethanol and prepare a 0.5wt% ethanol solution.
-Dip the base material into the coating solution for 1 min.
-Dry the coated material at room temperature or one hour at 50°C.

-Substrates-
-Lipidure®-CM can be coated on the following substrates:
-Plastics (PET, PMMA, PSt, PU, PC, PE, PP)
-Glass, Ceramics and Metals (SUS, titanium, gold)



-Comparison of results-

Protein Adsorption

Experimental procedure
-Dispensation of HRP-IgG conjugate solution into each well
-Incubation for 1 h at 25ºC
-Washing three times with Dulbeco’s PBS including 0.05% of Tween20.
-Dispensation of 3,3’,5,5’-tetramethylbenzidine solution
-Measurement of absorbance at 450 nm
The Lipidure® treatment was able to reduce the amount of IgG absorption to below 10% of non-treated pleat. The same results were observed with other proteins.


Macrophage adhesion

Experimental procedure
-Seeding of macrophage into each well (3.3 x 105 cells/well)
-Incubation for 1 h at 37ºC in 5% CO2 atmosphere
-Washing with Dulbeco’s PBS
-Fixing with glutaraldehyde solution
-Observation by SEM
The activation and adhesion of macrophage was prevented by Lipidure® treatment. Also, the Lipidure® treatment was effective for other cells.


-Coating-
PC surface can be made by coating with Lipidure®-CM. The following figure shows the relationship between “the number of times the coating was applied” and “the thickness of the coating film”. The thickness of the Lipidure®-CM coating film is calculated by weight measurement. These data are examples in the case of dip coating. The thickness was controlled by adjusting the number of times of a coating was applied and the concentration of Lipidure® in the coating solution.

-Safety Data-
We have the following toxicological data for Lipidure®-CM5206. Acute oral toxicity, Skin irritation, Skin sensitization, Mutagenic potential (Ames Test), Hemolysis test, Endotoxin test



Relationship between “number of times coating was applied” and “thickness of coating film”