Protein Assay

Protein assays determine the amount of protein in an unknown solution. Specially, Western blotting is widely used for detection of protein which is a target protein or not. In Western blotting, prior to protein immobilization on the PVDF or nitrocellulose membranes, sample proteins are separated using SDS polyacrylamide gel electrophoresis providing information about molecular weight and the potential existence of different isoforms of the proteins using monoclonal or polyclonal antibodies. For sensitive detection of target proteins, non-specific binding of monoclonal or polyclonal antibodies on the membrane should be reduced.

NOF has supplied additives, Biolipidure®, for suppression of non-specific binding of antibodies on the membranes. By using of Biolipidure®, high sensitive Western blotting would be done.

• Biolipidure®
• Reactive PCs
• Reactive MPC polymers