{"product_id":"lipidure-cm5206-1g","title":"Lipidure®-CM5206","description":"\u003cp\u003eIncoterms: EX-Works NOF's warehouse in Japan.\u003c\/p\u003e\n\u003cp\u003e***We will send you an email requesting your FedEx account number after receiving your order. ***\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0643\/8292\/6932\/files\/img_lipidure_cm_01.gif?v=1780052249\" alt=\"Coating Regents\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003e－Features－\u003c\/strong\u003e\u003cbr\u003e-Hemo-compatibility\u003cbr\u003e-Anti-adhesion of proteins and cells\u003cbr\u003e-Anti-denaturing of proteins\u003cbr\u003e-Anti-activation of cells\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e－Usage－\u003c\/strong\u003e\u003cbr\u003e-Dissolve Lipidure®-CM in ethanol and prepare a 0.5wt% ethanol solution.\u003cbr\u003e-Dip the base material into the coating solution for 1 min.\u003cbr\u003e-Dry the coated material at room temperature or one hour at 50°C. \u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e－Substrates－\u003c\/strong\u003e\u003cbr\u003e-Lipidure®-CM can be coated on the following substrates:\u003cbr\u003e-Plastics (PET, PMMA, PSt, PU, PC, PE, PP)\u003cbr\u003e-Glass, Ceramics and Metals (SUS, titanium, gold)\u003c\/p\u003e\n\u003chr\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0643\/8292\/6932\/files\/img_lipidure_cm_02.gif?v=1780052396\" alt=\"Structure of Lipidure CM5206\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003chr\u003e\n\u003cp\u003e\u003cstrong\u003e－Comparison of results－\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e\u003cspan style=\"color: #cc0000;\" color=\"#cc0000\"\u003eProtein Adsorption\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\" style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 49.018348%;\"\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0643\/8292\/6932\/files\/img_lipidure_cm_03.gif?v=1780052608\" alt=\"Protein Adsorption\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 50.981652%;\"\u003e\n\u003cstrong\u003eExperimental procedure\u003c\/strong\u003e\u003cbr\u003e-Dispensation of HRP-IgG conjugate solution into each well\u003cbr\u003e-Incubation for 1 h at 25ºC\u003cbr\u003e-Washing three times with Dulbeco’s PBS including 0.05% of Tween20. \u003cbr\u003e-Dispensation of 3,3’,5,5’-tetramethylbenzidine solution\u003cbr\u003e-Measurement of absorbance at 450 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"2\" style=\"width: 100%;\"\u003eThe Lipidure® treatment was able to reduce the amount of IgG absorption to below 10% of non-treated pleat. The same results were observed with other proteins.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003chr\u003e\n\u003cp\u003e\u003cbr\u003e\u003cstrong\u003e\u003cspan style=\"color: #cc0000;\" color=\"#cc0000\"\u003eMacrophage adhesion\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" cellpadding=\"0\" style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 49.803605%;\"\u003e\n\u003cimg\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0643\/8292\/6932\/files\/img_lipidure_cm_04.jpg?v=1780052695\" alt=\"Macrophage adhesion\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" style=\"width: 50.196395%;\"\u003e\n\u003cstrong\u003eExperimental procedure\u003c\/strong\u003e\u003cbr\u003e-Seeding of macrophage into each well (3.3 x 105 cells\/well) \u003cbr\u003e-Incubation for 1 h at 37ºC in 5% CO2 atmosphere\u003cbr\u003e-Washing with Dulbeco’s PBS \u003cbr\u003e-Fixing with glutaraldehyde solution\u003cbr\u003e-Observation by SEM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"2\" style=\"width: 100%;\"\u003eThe activation and adhesion of macrophage was prevented by Lipidure® treatment. Also, the Lipidure® treatment was effective for other cells.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003chr\u003e\n\u003cp\u003e\u003cbr\u003e\u003cstrong\u003e－Coating－\u003c\/strong\u003e\u003cbr\u003ePC surface can be made by coating with Lipidure®-CM. The following figure shows the relationship between “the number of times the coating was applied” and “the thickness of the coating film”. The thickness of the Lipidure®-CM coating film is calculated by weight measurement. These data are examples in the case of dip coating. The thickness was controlled by adjusting the number of times of a coating was applied and the concentration of Lipidure® in the coating solution.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e－Safety Data－\u003c\/strong\u003e\u003cbr\u003eWe have the following toxicological data for Lipidure®-CM5206. Acute oral toxicity, Skin irritation, Skin sensitization, Mutagenic potential (Ames Test), Hemolysis test, Endotoxin test.\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0643\/8292\/6932\/files\/img_lipidure_cm_05.gif?v=1780052757\" alt=\"Safety Data Chart\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp\u003eRelationship between “number of times coating was applied” and “thickness of coating film”\u003c\/p\u003e","brand":"NOF AMERICA","offers":[{"title":"Default Title","offer_id":42271142477908,"sku":null,"price":300.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0643\/8292\/6932\/files\/lipidure-cm5206.jpg?v=1766588142","url":"https:\/\/nofamerica.com\/products\/lipidure-cm5206-1g","provider":"NOF AMERICA CORPORATION","version":"1.0","type":"link"}